pSK226
(Plasmid #129268)

• Purpose: dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter
• Depositing Lab: Ahmad Khalil
• Publication: Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.

Backbone

• Vector backbone: pSK247
• Backbone manufacturer: Szilvia Kiriakov
• Total vector size (bp): 9928
• Modifications to backbone: LR reaction with pENTR-Rnq1
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rnq1
• Alt name: YCL028W
• Alt name: SGD:S000000533
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1215
• GenBank ID: NC_001135.5

Resource Information

• Supplemental Documents:
  • pSK226_KanMX_LEU2_Sup-Neon-37-12-RNQ1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the Rnq1 dual yTRAP sensor plasmid to be used in combination with pSK260. pSK226 should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418.