pCMV 3xFLAG-ATG3 K243R
(Plasmid
#129301)
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PurposeExpresses pCMV 3xFLAG-ATG3 K243R in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity College London
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 129301-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 129301-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV-tripleFLAG-Gateway
- Backbone size w/o insert (bp) 4711
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUbiquitin-like-conjugating enzyme ATG3
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SpeciesH. sapiens (human)
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Mutationchanged Lysine 243 to Arginine
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Entrez GeneATG3 (a.k.a. APG3, APG3-LIKE, APG3L, PC3-96, hApg3)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVF_pCDNA3
- 3′ sequencing primer hGH-PA-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 129301-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 129301-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV 3xFLAG-ATG3 K243R was a gift from Robin Ketteler (Addgene plasmid # 129301 ; http://n2t.net/addgene:129301 ; RRID:Addgene_129301) -
For your References section:
Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R. J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 10.1074/jbc.AC119.009977. 10.1074/jbc.AC119.009977 PubMed 31315929
