pTRIPdU3-MND-FLAG-FLI1-IRES-GFP
(Plasmid
#129496)
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PurposeLentiviral expression of human FLI1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTRIPdU3-MND-IRES-GFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLI1
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SpeciesH. sapiens (human)
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Entrez GeneFLI1 (a.k.a. BDPLT21, EWSR2, FLI-1, SIC-1)
- Promoter MND
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRIPdU3-MND-FLAG-FLI1-IRES-GFP was a gift from Marjorie Brand (Addgene plasmid # 129496 ; http://n2t.net/addgene:129496 ; RRID:Addgene_129496) -
For your References section:
Single-Cell Proteomics Reveal that Quantitative Changes in Co-expressed Lineage-Specific Transcription Factors Determine Cell Fate. Palii CG, Cheng Q, Gillespie MA, Shannon P, Mazurczyk M, Napolitani G, Price ND, Ranish JA, Morrissey E, Higgs DR, Brand M. Cell Stem Cell. 2019 May 2;24(5):812-820.e5. doi: 10.1016/j.stem.2019.02.006. Epub 2019 Mar 14. 10.1016/j.stem.2019.02.006 PubMed 30880026
