|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129628||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter CMV,&Rhamnose
/ Fusion Protein
- 6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to S]LT[V to D]AG[M to T]LGPSLLTPRRATAAQAATDAS (N terminal on insert)
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FHL-cpmTq2-Calcium-lifetime-sensor was a gift from Dorus Gadella (Addgene plasmid # 129628 ; http://n2t.net/addgene:129628 ; RRID:Addgene_129628)
For your References section:A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J , Puschhof J , de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ, Goedhart J. bioRxiv 10.1101/2021.06.21.449214