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(Plasmid #129628)


Item Catalog # Description Quantity Price (USD)
Plasmid 129628 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Promoter CMV,&Rhamnose
  • Tag / Fusion Protein
    • 6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to S]LT[V to D]AG[M to T]LGPSLLTPRRATAAQAATDAS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments


How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FHL-cpmTq2-Calcium-lifetime-sensor was a gift from Dorus Gadella (Addgene plasmid # 129628 ; ; RRID:Addgene_129628)
  • For your References section:

    A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J , Puschhof J , de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ, Goedhart J. bioRxiv 10.1101/2021.06.21.449214