PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
(Plasmid #129632)

Purpose

Visualization of Akt and ERK activity
Depositing Lab

Dorus Gadella
Publication

Chavez-Abiega et al bioRxiv 2021.07.27.453948 ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	129632	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
PiggyBac
(Search Vector Database)
Vector type
Mammalian Expression
Selectable markers
Puromycin

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
Species
Synthetic
Promoter CMV

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site Unknown (unknown if destroyed)
3′ cloning site Unknown (unknown if destroyed)

Resource Information

Article Citing this Plasmid
- 1 Reference

Terms and Licenses

Academic/Nonprofit Terms
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG was a gift from Dorus Gadella (Addgene plasmid # 129632 ; http://n2t.net/addgene:129632 ; RRID:Addgene_129632)
For your References section:

Heterogeneity and dynamics of ERK and Akt activation by G protein-coupled receptors depend on the activated heterotrimeric G proteins. Chavez-Abiega S, Gronloh M, Gadella TWJ, Bruggeman FJ, Goedhart J. bioRxiv 2021.07.27.453948 10.1101/2021.07.27.453948

PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG (Plasmid #129632)