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(Plasmid #129654)


Item Catalog # Description Quantity Price (USD)
Plasmid 129654 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8500
  • Modifications to backbone
    pT2ADW was constructed as follows: The D4Z4 insulator was inserted before the CAG promoter of pT2AL200R175 carrying Tol2 recombination sites. Then, the WPRE sequence of pCSII-EF was inserted before the poly A sequence.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Promoter CAG

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2ADW_2paRAF was a gift from Michiyuki Matsuda (Addgene plasmid # 129654 ; ; RRID:Addgene_129654)
  • For your References section:

    FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata S, Matsuda M. Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 10.1038/s41592-019-0541-5. 10.1038/s41592-019-0541-5 PubMed 31501546