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pCK354
(Plasmid #129698)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 129698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYC177
  • Backbone size w/o insert (bp) 3941
  • Total vector size (bp) 6059
  • Modifications to backbone
    Addition of two 1000bp sequences with homology to the Synechocystis sp. PCC6803 genome, allowing chromosomal integration. Introduction of the rhaBAD promoter from E. coli (allowing inducible expression with L-rhamnose). Introduction of a synthetic RBS and yfp as a reporter. Introduction of rhaS gene from E. coli, the transcriptional activator required for rhaBAD function in Synechocystis.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    PrhaBAD
  • Alt name
    promoter found 5' end of rhaBAD operon in E. coli
  • Species
    E. coli
  • Insert Size (bp)
    122

Gene/Insert 2

  • Gene/Insert name
    yfp
  • Alt name
    enhanced yellow fluorescent protein from A. victoria (BBa_E0030)
  • Species
    A. victoria
  • Insert Size (bp)
    720

Gene/Insert 3

  • Gene/Insert name
    rhaS
  • Alt name
    transcriptional activator of the rhaBAD promoter in E. coli with native E. coli RBS
  • Species
    E. coli
  • Insert Size (bp)
    837

Gene/Insert 4

  • Gene/Insert name
    Terminator ECK120010799
  • Species
    E. coli
  • Insert Size (bp)
    60

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK354 was a gift from John Heap (Addgene plasmid # 129698 ; http://n2t.net/addgene:129698 ; RRID:Addgene_129698)
  • For your References section:

    Transcriptional Terminators Allow Leak-Free Chromosomal Integration of Genetic Constructs in Cyanobacteria. Kelly CL, Taylor GM, Satkute A, Dekker L, Heap JT. Microorganisms. 2019 Aug 16;7(8). pii: microorganisms7080263. doi: 10.3390/microorganisms7080263. 10.3390/microorganisms7080263 PubMed 31426276