PurposeExpress HA-KASH for nuclei labeling and purification
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129704||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)663
- Promoter EF1a
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer CAAGCCTCAGACAGTGGTTCAAAG
- 3′ sequencing primer CACAAATTTTGTAATCCAGAGGTTG (Common Sequencing Primers)
Please note: Plasmid contains SFEF141-144GAAG change in the linker region of the GFP nanobody-KASH insert. These differences are not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE was a gift from Yi Zhang (Addgene plasmid # 129704 ; http://n2t.net/addgene:129704 ; RRID:Addgene_129704)
For your References section:In vivo nuclear capture and molecular profiling identifies Gmeb1 as a transcriptional regulator essential for dopamine neuron function. Tuesta LM, Djekidel MN, Chen R, Lu F, Wang W, Sabatini BL, Zhang Y. Nat Commun. 2019 Jun 7;10(1):2508. doi: 10.1038/s41467-019-10267-0. 10.1038/s41467-019-10267-0 PubMed 31175277