pAY02
(Plasmid
#13043)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET17xb
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMutS
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SpeciesE. coli
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Insert Size (bp)2562
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MutationT193TT to G193CT Phe to Ala mutation
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GenBank IDU69873
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
encodes mutant E.coli His6-MutS protein F36A (full length)
Derived from pTX412
Feng, G. and Winkler, M.E.
1995 Biotechniques 19:956-965
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAY02 was a gift from Peggy Hsieh (Addgene plasmid # 13043 ; http://n2t.net/addgene:13043 ; RRID:Addgene_13043) -
For your References section:
Requirement for Phe36 for DNA binding and mismatch repair by Escherichia coli MutS protein. Yamamoto A, Schofield MJ, Biswas I, Hsieh P. Nucleic Acids Res. 2000 Sep 15. 28(18):3564-9. 10.1093/nar/28.18.3564 PubMed 10982877