Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13044||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3600
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)3000
MutationPhe39Ala mutation Full length
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
Derived from pETMutS
Biswas, Il and Hsieh,P.
1996 JBC 271:5040-5048
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET23MutS-F39A was a gift from Peggy Hsieh (Addgene plasmid # 13044 ; http://n2t.net/addgene:13044 ; RRID:Addgene_13044)
For your References section:Photocross-linking of the NH2-terminal region of Taq MutS protein to the major groove of a heteroduplex DNA. Malkov VA, Biswas I, Camerini-Otero RD, Hsieh P. J Biol Chem. 1997 Sep 19. 272(38):23811-7. 10.1074/jbc.272.38.23811 PubMed 9295328