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pRK603
(Plasmid #8831)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8831 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZA31
  • Backbone manufacturer
    Herman Bujard Lab
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21-Pro
  • Growth instructions
    For best results, perform intracellular processing experiments at 30C.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TEV protease, S219D mutant
  • Alt name
    tobacco etch virus protease
  • Species
    tobacco etch virus
  • Insert Size (bp)
    700
  • Mutation
    S219D mutant

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pLTet-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATCC
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK603 was a gift from David Waugh (Addgene plasmid # 8831 ; http://n2t.net/addgene:8831 ; RRID:Addgene_8831)
  • For your References section:

    Controlled intracellular processing of fusion proteins by TEV protease. Kapust RB, Waugh DS. Protein Expr Purif. 2000 Jul . 19(2):312-8. 10.1006/prep.2000.1251 PubMed 10873547