|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8831||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerHerman Bujard Lab
- Backbone size w/o insert (bp) 6700
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsFor best results, perform intracellular processing experiments at 30C.
Copy numberLow Copy
Gene/Insert nameTEV protease, S219D mutant
Alt nametobacco etch virus protease
Speciestobacco etch virus
Insert Size (bp)700
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pLTet-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK603 was a gift from David Waugh (Addgene plasmid # 8831 ; http://n2t.net/addgene:8831 ; RRID:Addgene_8831)
For your References section:Controlled intracellular processing of fusion proteins by TEV protease. Kapust RB, Waugh DS. Protein Expr Purif. 2000 Jul . 19(2):312-8. 10.1006/prep.2000.1251 PubMed 10873547