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Depositing Lab
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Depositing OrganizationMayo Foundation for Medical Education and Research
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13051 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 13051-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 13051-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDsRed2-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAB5A (Canis familiaris)
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Alt nameDsRed-rab5 dominant negative
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SpeciesCanis familiaris
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Insert Size (bp)699
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Mutationasparagine 3 changed to serine (neutral); asparagine 133 changed to isoleucine (dominant negative); serine 210 changed to asparagine (neutral)
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GenBank IDNM_001003317
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Entrez GeneRAB5A
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Tags
/ Fusion Proteins
- DsRed2 (N terminal on backbone)
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer DsRed1-C
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bythe modified canine Rab5A was received from Cecilia Bucci (University of Lecce, Lecce, Italy)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 13051-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 13051-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DsRed-Rab5 DN was a gift from Richard Pagano (Addgene plasmid # 13051 ; http://n2t.net/addgene:13051 ; RRID:Addgene_13051) -
For your References section:
Glycosphingolipids internalized via caveolar-related endocytosis rapidly merge with the clathrin pathway in early endosomes and form microdomains for recycling. Sharma DK, Choudhury A, Singh RD, Wheatley CL, Marks DL, Pagano RE. J Biol Chem. 2003 Feb 28. 278(9):7564-72. 10.1074/jbc.M210457200 PubMed 12482757
