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DsRed-Rab5 WT
(Plasmid #13050)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13050 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDsRed2-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB5A (Canis familiaris)
  • Alt name
    DsRed-rab5 wild type
  • Species
    Canis familiaris
  • Insert Size (bp)
    699
  • Mutation
    asparagine 3 changed to serine (neutral); serine 210 changed to asparagine (neutral)
  • GenBank ID
    NM_001003317
  • Entrez Gene
    RAB5A
  • Tags / Fusion Proteins
    • DsRed2 (N terminal on backbone)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DsRed-Rab5 WT was a gift from Richard Pagano (Addgene plasmid # 13050 ; http://n2t.net/addgene:13050 ; RRID:Addgene_13050)
  • For your References section:

    Glycosphingolipids internalized via caveolar-related endocytosis rapidly merge with the clathrin pathway in early endosomes and form microdomains for recycling. Sharma DK, Choudhury A, Singh RD, Wheatley CL, Marks DL, Pagano RE. J Biol Chem. 2003 Feb 28. 278(9):7564-72. 10.1074/jbc.M210457200 PubMed 12482757