27 pCR plcg 1 CDS
Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13058||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3900
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Entrez Geneplcg1 (a.k.a. fj46f04, wu:fj46f04, wu:fj57a05)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 rev
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS.
For riboprobe, digest with MluI and use SP6 polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:27 pCR plcg 1 CDS was a gift from Nathan Lawson (Addgene plasmid # 13058 ; http://n2t.net/addgene:13058 ; RRID:Addgene_13058)
For your References section:phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Lawson ND, Mugford JW, Diamond BA, Weinstein BM. Genes Dev. 2003 Jun 1. 17(11):1346-51. 10.1101/gad.1072203 PubMed 12782653