|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||130748||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namePPARD R318K
SpeciesH. sapiens (human)
Entrez GenePPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pMSCVseqfw (CCCTTGAACCTCCTCGTTCGACC)
- 3′ sequencing primer pMSCVseqrv (GAGACGTGCTACTTCCATTTGTC) (Common Sequencing Primers)
Terms and Licenses
Generated by PCR mutagenesis. Please visit https://www.biorxiv.org/content/10.1101/525576v2 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCVbsd-PPARD-R318K was a gift from Till Adhikary (Addgene plasmid # 130748 ; http://n2t.net/addgene:130748 ; RRID:Addgene_130748)
For your References section:PPARβ/δ recruits NCOR and regulates transcription reinitiation. Legrand N, Bretscher CL, Zielke S, Wilke B, Daude M, Fritz B, Diederich WE, Adhikary T. BioRxiv 10.1101/525576