hTLR1 flag
(Plasmid #13081)

• Depositing Lab: Ruslan Medzhitov
• Publication: toll-like receptors (unpublished)

Backbone

• Vector backbone: pFlag-CMV-1
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TLR1
• Alt name: toll-like receptor 1
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Mutation: Amino acids 1-18 have been replaced with mammalian signal sequence (SALLILALVGAAVA) followed by Flag tag
• GenBank ID: NM_003263 AAY85640.1
• Entrez Gene: TLR1 (a.k.a. CD281, TIL, TIL. LPRS5, rsc786)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV30
• 3′ sequencing primer: CMV24

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results indicate that GenBank ID AAY85640.1 is better match for the human TLR1 insert sequence in this plasmid. When compared to GenBank ID NM_003263 there is an apparent mutation (R80T) which may represent a valid polymorphism.