Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13081||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nametoll-like receptor 1
SpeciesH. sapiens (human)
Insert Size (bp)2300
MutationAmino acids 1-18 have been replaced with mammalian signal sequence (SALLILALVGAAVA) followed by Flag tag
GenBank IDNM_003263 AAY85640.1
Entrez GeneTLR1 (a.k.a. CD281, TIL, TIL. LPRS5, rsc786)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV30
- 3′ sequencing primer CMV24 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing results indicate that GenBank ID AAY85640.1 is better match for the human TLR1 insert sequence in this plasmid. When compared to GenBank ID NM_003263 there is an apparent mutation (R80T) which may represent a valid polymorphism.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hTLR1 flag was a gift from Ruslan Medzhitov (Addgene plasmid # 13081 ; http://n2t.net/addgene:13081 ; RRID:Addgene_13081)