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pKIF1C-2xFLAG
(Plasmid #130979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130979 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 130979-D50 50 μg of DNA in Tris buffer $495
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DNA 130979-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF1C-2xFLAG
  • Alt name
    KIF1C kinesin family member 1C
  • Species
    H. sapiens (human)
  • Mutation
    4 silent mutations that make this construct resistant to RNAi oligo siKIF1C-1 also used in this paper. Nucleotides 649-663 (TCCCATGCCGTCTTT) have been replaced with TCGCACGCAGTCTTC.
  • Entrez Gene
    KIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKIF1C-2xFLAG was a gift from Anne Straube (Addgene plasmid # 130979 ; http://n2t.net/addgene:130979 ; RRID:Addgene_130979)

  • For your References section:

    Directional persistence of migrating cells requires Kif1C-mediated stabilization of trailing adhesions. Theisen U, Straube E, Straube A. Dev Cell. 2012 Dec 11;23(6):1153-66. doi: 10.1016/j.devcel.2012.11.005. 10.1016/j.devcel.2012.11.005 PubMed 23237952
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