pAAV-CaMKIIa-mScarlet
(Plasmid #131000)

Purpose

AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter
Depositing Lab

Karl Deisseroth
Publication

Marshel et al Science. 2019 Aug 9;365(6453). pii: science.aaw5202. doi: 10.1126/science.aaw5202. Epub 2019 Jul 18. ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	131000	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pAAV-MCS
(Search Vector Database)
Backbone manufacturer
Stratagene
Vector type
AAV

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
High Copy

Gene/Insert

Gene/Insert name
mScarlet
Species
Synthetic
Promoter CaMKIIa

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site BamHI (not destroyed)
3′ cloning site EcoRI (not destroyed)
5′ sequencing primer CTGGATGCTGACGAAGGCTCG
3′ sequencing primer GAATACCAGTCAATCTTTCAC

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- pAAV-CaMKIIa-mScarlett-WPRE.gb

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pAAV-CaMKIIa-mScarlet was a gift from Karl Deisseroth (Addgene plasmid # 131000 ; http://n2t.net/addgene:131000 ; RRID:Addgene_131000)
For your References section:

Cortical layer-specific critical dynamics triggering perception. Marshel JH, Kim YS, Machado TA, Quirin S, Benson B, Kadmon J, Raja C, Chibukhchyan A, Ramakrishnan C, Inoue M, Shane JC, McKnight DJ, Yoshizawa S, Kato HE, Ganguli S, Deisseroth K. Science. 2019 Aug 9;365(6453). pii: science.aaw5202. doi: 10.1126/science.aaw5202. Epub 2019 Jul 18. 10.1126/science.aaw5202 PubMed 31320556

pAAV-CaMKIIa-mScarlet (Plasmid #131000)