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pX458M-53BP1-DN1S
(Plasmid #131045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131045 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 131045-D50 50 μg of DNA in Tris buffer $495
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DNA 131045-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    PX458M
  • Backbone size w/o insert (bp) 9299
  • Total vector size (bp) 10583
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpCas9-53BP1-DN1S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1284
  • Mutation
    Only expressing residues 1231-1644 of complete 53BP1 CDS.
  • GenBank ID
    NM_005657.2
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Promoter CBH
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • SpCas9 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SanDI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer GAT CAC AAA GCA CGT GGC ACA G
  • 3′ sequencing primer GAA CAG CTC CTC GCC CTT GC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX458M-53BP1-DN1S was a gift from Punam Malik (Addgene plasmid # 131045 ; http://n2t.net/addgene:131045 ; RRID:Addgene_131045)

  • For your References section:

    CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites. Jayavaradhan R, Pillis DM, Goodman M, Zhang F, Zhang Y, Andreassen PR, Malik P. Nat Commun. 2019 Jun 28;10(1):2866. doi: 10.1038/s41467-019-10735-7. 10.1038/s41467-019-10735-7 PubMed 31253785
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