pANTO3
(Plasmid #131107)

• Purpose: To generate a replicative plasmid carrying mycobacteriophage L5 integrase
• Depositing Lab: Riccardo Manganelli
• Publication: Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1038/s41598-019-42319-2.

Backbone

• Vector backbone: pMV261 plasmid
• Backbone size w/o insert (bp): 3920
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mycobacteriophage L5 integrase
• Species: temperate phage of the mycobacteria
• Insert Size (bp): 2000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid has the gene for mycobacteriophage L5 integrase belonging to pMV306 plasmid (Stover CK et al. (1991). Nature 351: 456–460).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence for the vector is that of pMV261 after digestion using NheI- KpnI.