pAc-Cdc3-Cdc10-SNAP
(Plasmid
#131161)
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PurposeExpression of Septin rods
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepACYC-Duet1
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Backbone manufacturerNovagen
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Vector typeBacterial Expression ; bicistronic
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCdc3 + Cdc10-SNAP
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SpeciesS. cerevisiae (budding yeast)
- Promoter T7
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Tag
/ Fusion Protein
- Cdc10: SNAP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
combine with pET-Cdc12-Cdc11 for expression of SNAP-tagged septin rods
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc-Cdc3-Cdc10-SNAP was a gift from Nils Johnsson (Addgene plasmid # 131161 ; http://n2t.net/addgene:131161 ; RRID:Addgene_131161) -
For your References section:
An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation. Renz C, Johnsson N, Gronemeyer T. BMC Biotechnol. 2013 Jul 26;13:60. doi: 10.1186/1472-6750-13-60. 10.1186/1472-6750-13-60 PubMed 23889817
