pTSN6
(Plasmid #131318)

• Purpose: Integrating the full-length tetO array (from pLAU44) near the his-3 gene in N. crassa
• Depositing Lab: Eugene Gladyshev
• Publication: Nguyen et al Fungal Genet Biol. 2020 Mar;136:103316. doi: 10.1016/j.fgb.2019.103316. Epub 2019 Dec 9.

Backbone

• Vector backbone: pMF272
• Backbone size w/o insert (bp): 6803
• Total vector size (bp): 15736
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Gentamicin, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Full-length tetO array (XbaI-NheI fragment of pLAU44)
• Species: Synthetic
• Insert Size (bp): 8933

Resource Information

• A portion of this plasmid was derived from a plasmid made by: doi: 10.1046/j.1365-2958.2003.03640.x

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TetO arrays are unstable and dual selection with Ampicillin and Gentamycin are essential for the maintenance of the array. Confirm full-length plasmid by restriction digest and Sanger sequencing of any clones prior to use.

Some minor sequence discrepancies located in the TetO repeat array exist between Sanger and NGS based data. These are not of functional concern for use of the plasmid.