EcNGsC-S308V-pKK223
(Plasmid #131393)

• Purpose: Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.
• Depositing Lab: Martin Horvath
• Publication: Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639.

Backbone

• Vector backbone: pKK223
• Backbone size w/o insert (bp): 4577
• Total vector size (bp): 5663
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EcNGsC MutY chimera S308V
• Alt name: S308V EcNGsC
• Alt name: S308V
• Species: E. coli and Geobacillus stearothermophilus
• Insert Size (bp): 1086
• Mutation: Fusion of two MutY genes. Residues 1-225 are from E.coli MutY and residues 226-360 correspond with residues 231-365 of G.stearothermophilus MutY. Amino acid change S308V in Gs MutY, corresponding with position 303 in the chimera MutY.
• Promoter: tac

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: UpTac - GTTTTTTGCGCCGACATCATAACGGTTC
• 3′ sequencing primer: TacTrm - GCTTCTGCGTTCTGATTTAATCTGTATCAGGCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry