pIRES2-smGFP-Kv2.1
(Plasmid #131709)

• Purpose: Expresses Kv2.1 in mammalian cells
• Depositing Lab: Jennifer Kearney
• Publication: Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi: 10.1002/ana.25607. Epub 2019 Oct 24.

Backbone

• Vector backbone: pIRES2-DsRed-Express
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 7982
• Modifications to backbone: DsRed-Express was replaced with smGFP by Gibson Assembly
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KCNB1
• Alt name: potassium voltage-gated channel subfamily B member 1
• Alt name: Kv2.1
• Species: H. sapiens (human)
• GenBank ID: NM_004975
• Entrez Gene: KCNB1 (a.k.a. DRK1, Kv2.1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: NheI (destroyed during cloning)
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: dsRed1_N_primer

Resource Information

• Supplemental Documents:
  • pIRES2smGFP-KV2.1-WT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry