|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||132112||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 2550
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt nameVGAT, bA122O1.1, VIAAT
SpeciesH. sapiens (human)
Insert Size (bp)1575
Entrez GeneSLC32A1 (a.k.a. VGAT, VIAAT)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
For more information on the full Resolute plasmid collection, please see www.addgene.org/depositor-collections/re-solute/. Please provide your feedback on this plasmid to the RESOLUTE consortium by filling out their RESOLUTE repository feedback form: https://forms.office.com/Pages/ResponsePage.aspx?id=0e05yklzmkS7rjFGQL4N7z4feCLQvEJAmVcOCM_u885UN1JJRko0Ukg4TVQwNTZLOUxPQVJWT1NHUCQlQCN0PWcu
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR221_SLC32A1 was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 132112 ; http://n2t.net/addgene:132112 ; RRID:Addgene_132112)