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pDONR221_SLC39A10
(Plasmid #132275)

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Item Catalog # Description Quantity Price (USD)
Plasmid 132275 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 132275-D50 50 μg of DNA in Tris buffer $495 *
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DNA 132275-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 2550
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC39A10
  • Alt name
    KIAA1265, FLJ90515, DKFZp564L2123
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2493
  • Entrez Gene
    SLC39A10 (a.k.a. LZT-Hs2, MGC126565, MGC138428, DKFZp781L10106)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on the full Resolute plasmid collection, please see www.addgene.org/depositor-collections/re-solute/. Please provide your feedback on this plasmid to the RESOLUTE consortium by filling out their RESOLUTE repository feedback form: https://forms.office.com/Pages/ResponsePage.aspx?id=0e05yklzmkS7rjFGQL4N7z4feCLQvEJAmVcOCM_u885UN1JJRko0Ukg4TVQwNTZLOUxPQVJWT1NHUCQlQCN0PWcu

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221_SLC39A10 was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 132275 ; http://n2t.net/addgene:132275 ; RRID:Addgene_132275)

  • For your References section:

    Functional characterization of SLC39 family members ZIP5 and ZIP10 in overexpressing HEK293 cells reveals selective copper transport activity. Polesel M, Ingles-Prieto A, Christodoulaki E, Ferrada E, Doucerain C, Altermatt P, Knecht M, Kuhn M, Steck AL, Wilhelm M, Manolova V. Biometals. 2022 Dec 1. doi: 10.1007/s10534-022-00474-6. 10.1007/s10534-022-00474-6 PubMed 36454509
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