|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||132334||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesCas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana)
MutationCodon optimize for expression and stability in Zea mays
Entrez GeneCBF1 (a.k.a. AT4G25490, ATCBF1, C-repeat/DRE binding factor 1, DRE BINDING PROTEIN 1B, DREB1B, T30C3.11, TRANSCRIPTIONAL ACTIVATOR CBF1)
/ Fusion Protein
- AT-CBF1 (C terminal on insert)
- Cloning method Gibson Cloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PV225 was a gift from Joshua Young (Addgene plasmid # 132334 ; http://n2t.net/addgene:132334 ; RRID:Addgene_132334)
For your References section:Repurposing of Type I-E CRISPR-Cascade for gene activation in plants. Joshua K. Young, Stephen L. Gasior, Spencer Jones, Lijuan Wang, Pedro Navarro, Becca Vickroy & Rodolphe Barrangou. Nature Communications Biology 10.1038/s42003-019-0637-6