Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
(Plasmid #132409)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 132409 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE-EF1a-IRES-puro
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dRanCas13b
  • Species
    Ruminoccocus flavefaciens XPD3002
  • Mutation
    H147A; H1044A
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is prone to recombination. Test multiple colonies to ensure the full plasmid is isolated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag was a gift from Ling-Ling Chen (Addgene plasmid # 132409 ; http://n2t.net/addgene:132409 ; RRID:Addgene_132409)
  • For your References section:

    Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems. Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen LL. Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19. 10.1016/j.molcel.2019.10.024 PubMed 31757757