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Addgene

pspCas9-HF-tetra-com-vector
(Plasmid #132555)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132555 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDNA3,1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    High fidelity Cas9
  • Species
    Synthetic

Gene/Insert 2

  • Gene/Insert name
    sgRNA scaffold with com replacing the Tetraloop
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pspCas9-HF-tetra-com-vector was a gift from Baisong Lu (Addgene plasmid # 132555 ; http://n2t.net/addgene:132555 ; RRID:Addgene_132555)
  • For your References section:

    Lentiviral Capsid-Mediated Streptococcus pyogenes Cas9 Ribonucleoprotein Delivery for Efficient and Safe Multiplex Genome Editing. Lu Z, Yao X, Lyu P, Yadav M, Yoo K, Atala A, Lu B. CRISPR J. 2021 Mar 16. doi: 10.1089/crispr.2020.0106. 10.1089/crispr.2020.0106 PubMed 33733873