GFP-NPM NLS2D
(Plasmid #13285)

• Depositing Lab: Xin Wang
• Publication: Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NPM
• Alt name: B23.1
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: Deletion of aa150-aa155
• Entrez Gene: NPM1 (a.k.a. B23, NPM)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: K. Nagata (U of Tsukuba, Japan)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NLS2D=nuclear localization signal deletion