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Sox2-SE-RGM-eGFP targeting vector
(Plasmid #133045)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133045 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sox2-SE-5arm-RGM-Sox2-SE-3arm
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer caggaaacagctatgac
  • 3′ sequencing primer gtaaaacgacggccag
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sox2-SE-RGM-eGFP targeting vector was a gift from Rudolf Jaenisch (Addgene plasmid # 133045 ; http://n2t.net/addgene:133045 ; RRID:Addgene_133045)
  • For your References section:

    Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Song Y, van den Berg PR, Markoulaki S, Soldner F, Dall'Agnese A, Henninger JE, Drotar J, Rosenau N, Cohen MA, Young RA, Semrau S, Stelzer Y, Jaenisch R. Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 10.1016/j.molcel.2019.06.045. 10.1016/j.molcel.2019.06.045 PubMed 31422875