pET sfGFP(150TAG)
(Plasmid
#133455)
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Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
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Insert Size (bp)744
- Promoter T7
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Tag
/ Fusion Protein
- His
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CCGATTCTGGTGGAACTG
- 3′ sequencing primer TAGGTCAGGGTGGTCAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET sfGFP(150TAG) was a gift from Yu-Hsuan Tsai (Addgene plasmid # 133455 ; http://n2t.net/addgene:133455 ; RRID:Addgene_133455) -
For your References section:
Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. Williams TL, Iskandar DJ, Nodling AR, Tan Y, Luk LYP, Tsai YH. Amino Acids. 2021 Jan;53(1):89-96. doi: 10.1007/s00726-020-02927-z. Epub 2020 Dec 17. 10.1007/s00726-020-02927-z PubMed 33331978
