pcDNA3-HA-VprBP (C590)
(Plasmid
#133589)
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PurposeExpresses human VprBP C590 mutant in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5462
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVpr (HIV-1) binding protein
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Alt nameVprBP
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Alt nameKIAA0800
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Alt nameDCAF1
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SpeciesH. sapiens (human)
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Insert Size (bp)4542
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MutationC590 mutation of human VprBP lacking the N-terminal portion involved in TET2 binding
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GenBank IDNM_014703
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Entrez GeneDCAF1 (a.k.a. RIP, VPRBP)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-VprBP (C590) was a gift from Yue Xiong (Addgene plasmid # 133589 ; http://n2t.net/addgene:133589 ; RRID:Addgene_133589) -
For your References section:
CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases. Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, Fan HY, Chen X, Xiong Y. Mol Cell. 2015 Jan 22;57(2):247-60. doi: 10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31. 10.1016/j.molcel.2014.12.002 PubMed 25557551
