Cry2PHR.mCh.CofS3E
(Plasmid
#133619)
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PurposeExpresses Cry2PHR - mCherry - CofilinS3E protein fusion
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Depositing Lab
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Depositing OrganizationEast Carolina University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 133619-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 133619-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemCherry - N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCry2PHR - mCherry - CofilinS3E
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SpeciesM. musculus (mouse), A. thaliana (mustard weed)
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Insert Size (bp)2745
- Promoter cmv
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site xhoI (not destroyed)
- 3′ cloning site notI (not destroyed)
- 5′ sequencing primer cmvF
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/857706v2 for bioRxiv preprint.
DNA (Catalog # 133619-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 133619-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cry2PHR.mCh.CofS3E was a gift from Robert Hughes (Addgene plasmid # 133619 ; http://n2t.net/addgene:133619 ; RRID:Addgene_133619) -
For your References section:
CofActor: A light- and stress-gated optogenetic clustering tool to study disease-associated cytoskeletal dynamics in living cells. Salem FB, Bunner WP, Prabhu VV, Kuyateh AB, O'Bryant CT, Murashov AK, Szatmari EM, Hughes RM. J Biol Chem. 2020 Aug 7;295(32):11231-11245. doi: 10.1074/jbc.RA119.012427. Epub 2020 May 18. 10.1074/jbc.RA119.012427 PubMed 32424038
