pET28a-MinC
(Plasmid
#133628)
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PurposeExpression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5245
- Total vector size (bp) 6034
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHis-MinC
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SpeciesE. coli
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Insert Size (bp)789
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Entrez GeneminC (a.k.a. b1176, ECK1164, minB)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMinC was obtained from plasmid pZH101 originally from D. RayChaudhuri.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-MinC was a gift from Petra Schwille (Addgene plasmid # 133628 ; http://n2t.net/addgene:133628 ; RRID:Addgene_133628) -
For your References section:
Min protein patterns emerge from rapid rebinding and membrane interaction of MinE. Loose M, Fischer-Friedrich E, Herold C, Kruse K, Schwille P. Nat Struct Mol Biol. 2011 May;18(5):577-83. doi: 10.1038/nsmb.2037. Epub 2011 Apr 24. 10.1038/nsmb.2037 PubMed 21516096
Map uploaded by the depositor.
