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pGL2-basic mt E2F-luciferase
(Plasmid #133708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133708 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 133708-D50 50 μg of DNA in Tris buffer $495
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DNA 133708-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL2-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Mammalian Expression ; trxn factor bind site

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E2F1
  • Species
    H. sapiens (human)
  • Mutation
    the three binding site copies are mutated form
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: HsBS00017288. TATA oligo is GATCTACTTGGGCATAAAGGCAGAGCAGGGCAGCTGCTGCTTACACTTA; 3xE2F-MUTANT oligo is CTGCAATTGCTCGACCAACTTGTGCAATTGCTCGACCAACTTGTGCAATTGCTCGACCAACTTGC.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-basic mt E2F-luciferase was a gift from James A. DeCaprio (Addgene plasmid # 133708 ; http://n2t.net/addgene:133708 ; RRID:Addgene_133708)

  • For your References section:

    The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins. Zalvide J, Stubdal H, DeCaprio JA. Mol Cell Biol 1998 Mar;18(3):1408-15. PubMed 9488456
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