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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4693
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Vector typeMammalian Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namevon Hippel-Lindau
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Alt nameVHL
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SpeciesR. norvegicus (rat)
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Insert Size (bp)572
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GenBank IDNM_052801
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Entrez GeneVhl (a.k.a. Vhlh)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer tctcgagctcaagcttcgg
- 3′ sequencing primer tgtggtatggctgattatgatcagtt (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMichael Lerman, NCI-Frederick
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-wtVHL was a gift from Lucy Anderson (Addgene plasmid # 13390 ; http://n2t.net/addgene:13390 ; RRID:Addgene_13390) -
For your References section:
The von Hippel-Lindau tumor suppressor targets to mitochondria. Shiao YH, Resau JH, Nagashima K, Anderson LM, Ramakrishna G. Cancer Res. 2000 Jun 1. 60(11):2816-9. PubMed 10850420
