pCI-Myc3-HPS4
(Plasmid
#133931)
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PurposeExpresses 3xMyc-HPS4 construct in mammalian cells
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 133931-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 133931-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCINeo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 7707
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPS4
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SpeciesH. sapiens (human)
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Insert Size (bp)2235
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MutationGlutamic acid 229 to Glycine; Valine 552 to Methionine ; Histidine 606 to Tyrosine; Glutamine 625 to Histidine
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GenBank IDNM_022081
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Entrez GeneHPS4 (a.k.a. BLOC3S2, LE)
- Promoter CMV I.E.
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Tag
/ Fusion Protein
- 3xMyc tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer SV40pA reverse
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 133931-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 133931-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Myc3-HPS4 was a gift from Juan Bonifacino (Addgene plasmid # 133931 ; http://n2t.net/addgene:133931 ; RRID:Addgene_133931) -
For your References section:
BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. Martina JA, Moriyama K, Bonifacino JS. J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 10.1074/jbc.M301294200. Epub 2003 May 19. 10.1074/jbc.M301294200 PubMed 12756248
