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pIW028
(Plasmid #134125)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 134125 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET3aTr
  • Backbone manufacturer
    Song Tan (Addgene plasmid # 64008)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His-Precission-human Ska1 MTBD
  • Species
    H. sapiens (human)
  • Mutation
    aa 132-255 and K183A K184A
  • Entrez Gene
    SKA1 (a.k.a. C18orf24)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIW028 was a gift from Iain Cheeseman (Addgene plasmid # 134125 ; http://n2t.net/addgene:134125 ; RRID:Addgene_134125)
  • For your References section:

    Microtubule Tip Tracking by the Spindle and Kinetochore Protein Ska1 Requires Diverse Tubulin-Interacting Surfaces. Monda JK, Whitney IP, Tarasovetc EV, Wilson-Kubalek E, Milligan RA, Grishchuk EL, Cheeseman IM. Curr Biol. 2017 Dec 4;27(23):3666-3675.e6. doi: 10.1016/j.cub.2017.10.018. Epub 2017 Nov 16. 10.1016/j.cub.2017.10.018 PubMed 29153323