Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13417||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUpstream region of mouse Fbx15 gene
SpeciesM. musculus (mouse)
Insert Size (bp)20
MutationMutated in Oct4 binding site
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer pGV-2U (Common Sequencing Primers)
The sequence of primer pGV-2U is ACT AAC ATA CGC TCT CCA TCA A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFbx15(-544/-527)-TK-Luc/Oct-m was a gift from Shinya Yamanaka (Addgene plasmid # 13417 ; http://n2t.net/addgene:13417 ; RRID:Addgene_13417)
For your References section:Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S. Mol Cell Biol. 2003 Apr . 23(8):2699-708. 10.1128/MCB.23.8.2699-2708.2003 PubMed 12665572
Map uploaded by the depositor.