PurposeB6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||134321||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerSen Wu, Capecchi laboratory
Vector typeMouse Targeting
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
SpeciesM. musculus (mouse)
/ Fusion Protein
- C-terminal fusion of T2A-tandem dimer-superfolder GFP
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Mice available from The Jackson Laboratory (https://www.jax.org/strain/036458).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB6-Cdk6-T2A-td-sfGFP-FNF-TK was a gift from Mario Capecchi (Addgene plasmid # 134321 ; http://n2t.net/addgene:134321 ; RRID:Addgene_134321)
For your References section:Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Nam HS, Capecchi MR. Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5. 10.1186/s13064-020-00139-5 PubMed 32183906