mApple-M1 SpastinHFDD
(Plasmid
#134462)
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PurposeM1 SpastinHFDD expression
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Depositing Lab
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Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134462 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 134462-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 134462-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemApple-C1
- Total vector size (bp) 6540
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM1 Spastin
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SpeciesH. sapiens (human)
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MutationMet to Ala at amino acid 87; His to Asp at amino acid 120; Phe to Asp at amino acid 124
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GenBank IDNM_014946
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Entrez GeneSPAST (a.k.a. ADPSP, FSP2, SPG4)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 134462-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 134462-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mApple-M1 SpastinHFDD was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 134462 ; http://n2t.net/addgene:134462 ; RRID:Addgene_134462) -
For your References section:
Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. Chang CL, Weigel AV, Ioannou MS, Pasolli HA, Xu CS, Peale DR, Shtengel G, Freeman M, Hess HF, Blackstone C, Lippincott-Schwartz J. J Cell Biol. 2019 Aug 5;218(8):2583-2599. doi: 10.1083/jcb.201902061. Epub 2019 Jun 21. 10.1083/jcb.201902061 PubMed 31227594
