pENTR1A DHFR.dn-cHSF1
(Plasmid
#134726)
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PurposeGateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepENTR1A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2278
- Total vector size (bp) 3871
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Vector typeEntry Vector for Gateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDHFR.dn-cHSF1
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SpeciesH. sapiens (human)
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Insert Size (bp)1593
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GenBank IDNM_005526.4
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Entrez GeneHSF1 (a.k.a. HSTF1)
- Promoter None
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Tag
/ Fusion Protein
- DHFR (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CTACAAACTCTTCCTGTTAGTTAG
- 3′ sequencing primer GAGACACGGGCCAGAGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR1A DHFR.dn-cHSF1 was a gift from Matthew D. Shoulders (Addgene plasmid # 134726 ; http://n2t.net/addgene:134726 ; RRID:Addgene_134726) -
For your References section:
Transportable, Chemical Genetic Methodology for the Small Molecule-Mediated Inhibition of Heat Shock Factor 1. Moore CL, Dewal MB, Nekongo EE, Santiago S, Lu NB, Levine SS, Shoulders MD. ACS Chem Biol. 2016 Jan 15;11(1):200-10. doi: 10.1021/acschembio.5b00740. Epub 2015 Nov 19. 10.1021/acschembio.5b00740 PubMed 26502114
Map uploaded by the depositor.
