pTF101.1gw1
(Plasmid
#134768)
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Purpose(Empty Backbone) A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation
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Depositing Lab
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Depositing OrganizationIowa State University
Located in United States of America -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTF101.1
- Backbone size (bp) 9189
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTF101.1gw1 was a gift from Kan Wang (Addgene plasmid # 134768 ; http://n2t.net/addgene:134768 ; RRID:Addgene_134768) -
For your References section:
Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Paz, M.M., Shou, H., Guo, Z., Zhang, Z., Banerjee, A., Wang, K. Euphytica (2004) 136: 167. 10.1023/B:EUPH.0000030669.75809.dc
