Lentiviral-His-tagged-PSPH
(Plasmid #134786)

• Purpose: Stable overexpression lentiviral vector of His tagged PSPH
• Depositing Lab: Seon-Young Kim
• Publication: Park et al Mol Cells. 2019 Aug 31;42(8):604-616. doi: 10.14348/molcells.2019.0160.

Backbone

• Vector backbone: pCDH-CMV-MCS-EF1-Puro
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7377
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Phosphoserine phosphatase
• Alt name: PSPH
• Species: H. sapiens (human)
• Entrez Gene: PSPH (a.k.a. PSP, PSPHD)
• Promoter: CMV
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry