pWay21-MKP-1 ASA
(Plasmid
#13479)
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Depositing Lab
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Depositing OrganizationYale University
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 13479-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 13479-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepWay21 EGFP
- Backbone size w/o insert (bp) 5800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMKP-1ASA
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Alt nameMKP-1FL ASA
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Alt nameMKP-1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1130
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MutationChanged Arginine 53 to Alanine, Changed Arginine 54 to Serine, Changed Arginine 55 to Alanine
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GenBank IDNM_013642
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Entrez GeneDusp1 (a.k.a. 3CH134, MKP1, Ptpn16, erp, mkp-1)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sma I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 13479-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 13479-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWay21-MKP-1 ASA was a gift from Anton Bennett (Addgene plasmid # 13479 ; http://n2t.net/addgene:13479 ; RRID:Addgene_13479) -
For your References section:
The noncatalytic amino terminus of mitogen-activated protein kinase phosphatase 1 directs nuclear targeting and serum response element transcriptional regulation. Wu JJ, Zhang L, Bennett AM. Mol Cell Biol. 2005 Jun . 25(11):4792-803. 10.1128/MCB.25.11.4792-4803.2005 PubMed 15899879