pED14xQ-1
(Plasmid #134794)

• Purpose: IPTG inducible expression of Q-TAGA qtRNA
• Depositing Lab: Ahmed Badran
• Publication: DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y.

Backbone

• Vector backbone: RK2 SpecR
• Total vector size (bp): 3240
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow in glucose to suppress expression of qtRNA
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Q-TAGA qtRNA
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pED14xQ-1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.