pFL-EGFP-3xFlag-DEST
(Plasmid
#134903)
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Purpose(Empty Backbone) Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastbac-Dual
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Backbone manufacturerInvitrogen
- Backbone size (bp) 5100
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Modifications to backboneinsertion of EGFP and gateway cassette for separate expression of EGFP (from p10 promoter) and 3x-Flag tagged target protein in baculovirus expression system
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Vector typeInsect Expression
- Promoter polyhedron
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Selectable markersGentamycin
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Tag
/ Fusion Protein
- 3x-Flag (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsAlso contains gentamcyin resistance gene that is transferred to Baculovirus genome using DH10Bac transformation.
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer pH
- 3′ sequencing primer SV40polyA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFL-EGFP-3xFlag-DEST was a gift from Andrew Deans (Addgene plasmid # 134903 ; http://n2t.net/addgene:134903 ; RRID:Addgene_134903) -
For your References section:
Branchpoint translocation by fork remodelers as a general mechanism of R-loop removal. Hodson C, van Twest S, Dylewska M, O'Rourke JJ, Tan W, Murphy VJ, Walia M, Abbouche L, Nieminuszczy J, Dunn E, Bythell-Douglas R, Heierhorst J, Niedzwiedz W, Deans AJ. Cell Rep. 2022 Dec 6;41(10):111749. doi: 10.1016/j.celrep.2022.111749. 10.1016/j.celrep.2022.111749 PubMed 36476850
Map uploaded by the depositor.
