GEF-H1 FLARE191.pa
(Plasmid #134931)

• Purpose: GEF-H1 biosensor that has GEF activity.
• Depositing Lab: Klaus Hahn
• Publication: Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097. doi: 10.1083/jcb.201812073. Epub 2019 Aug 16.

Backbone

• Vector backbone: pTriEx-4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 10000
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
• Alt name: GEF-H1 Flare 5.pa
• Species: H. sapiens (human)
• Insert Size (bp): 4578
• Promoter: CMV and T7
• Tag / Fusion Protein:
  • None.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: T7 Term

Resource Information

• Supplemental Documents:
  • GEF-H1 FLARE191

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry