C-12-BE
(Plasmid
#135040)
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PurposeC-T base editing using LjCDA1L2_1 fused to C terminal of nCas9
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Depositing Labs
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Depositing OrganizationCAS Center for Excellence in Brain Science and Intelligence Technology (INACTIVE)
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 135040-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 135040-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePx330
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Modifications to backbonesynthesized UGI-2A-EGFP cassette added to vector
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLjCDA1L2_1
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SpeciesLamprey
- Promoter U6 with EF-1alpha intron
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 135040-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 135040-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C-12-BE was a gift from Tian-Lin Cheng & Zilong Qiu (Addgene plasmid # 135040 ; http://n2t.net/addgene:135040 ; RRID:Addgene_135040) -
For your References section:
Expanding C-T base editing toolkit with diversified cytidine deaminases. Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. 10.1038/s41467-019-11562-6 PubMed 31399578
